Siai Zhang


Modulating the nisin expression system


Area: expression
Project Start: 1.06.2013
Supervisor(s): Prof. Dr. Lutz Schmitt, Prof. Dr. Sander Smits
University: Duesseldorf


Project overview

It has been demonstrated that thioether-bridged peptides would exhibit more resistance against the peptidases, enhancing their bioavailability. To understand the mechanism of thioether bridge-installing enzymes, such as NisC, we conduct a structural study on NisC in complex with prenisin (AC complex). Until now, the AC complex was successfully formed in our lab. The co-crystallization trials for the AC complex are currently ongoing. Furthermore, initial crystals of single NisC, required for crystallization soaking, was achieved.


Siai’s project
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