Dr. Mattjs Julsing

University

Dortmund

department: BCI, Laboratory of Chemical Biotechnology

More

TU Dortmund University, Department of Biochemical and Chemical Engineering, Laboratory of Chemical Biotechnology, Applied Biocatalysis Group

Research Interests

Oxygenase catalysis based on recombinant microorganisms

Oxidoreductases catalyze a large variety of regio-, stereo-, and chemoselective hydrocarbon oxyfunctionalizations - reactions which are important in industrial organic synthesis but difficult to achieve by chemical means. The especially versatile enzyme class of oxygenases is capable of specifically introducing oxygen from molecular oxygen into a large range of organic molecules under mild conditions. Thus, oxygenase catalysis is of utmost interest for the chemical and pharmaceutical industies.

The development of oxygenase-based bioprocesses suitable for industry faces hurdles that are not experienced with easy-to-use enzymes such as hydrolases, isomerases, or lyases. Oxygenases are often unstable, consist of multiple components, some of which might be membrane-bound, and require costly cofactors such as NAD(P)H. For synthetic applications on medium- to large-scale, the most promising approach is the use of oxygenase containing whole microbial cells, which regenerate cofactors and continuously synthesize the oxygenase.

Based on Escherichia coli, Pseudomonas and yeast strains, we develop recombinant whole-cell catalysts and processes for oxygenase catalysis. For several different oxygenase classes, which contain heme, non-heme iron, or flavin in the active site, we investigate critical issues such as low enzyme activity and specificity, product degradation, cofactor recycling, reactant toxicity, and substrate and oxygen mass transfer. To overcome them, we use both biochemical process engineering and biocatalyst engineering approaches.

To find out more, visit the website of the Applied Biocatalysis Group.

Students (co-)supervised

Kerstin Lange