Sergej Wendler

Project

Proteome studies on the synthesis and mode of action of the medically important secondary metabolite acarbose from Actinoplanes utahensis sp. SE50/110

Info

Area: polyomics
Project Start: 1.10.2010
Supervisor(s): Dr. Jörn Kalinowski, Prof. Dr. Karsten Niehaus, Prof. Dr. Alfred Pühler
University: Bielefeld

More

Sergej was an associate student of the CLIB-Graduate Cluster. He successfully defended his thesis in May 2015 and received his doctorate degree from Bielefeld University.

Project overview

The α-glycosidase inhibitor acarbose is a bacterial secondary metabolite that is used for the treatment of Diabetes mellitus type II. A natural producer of acarbose is Actinoplanes sp. SE50/110, a gram-positive, aerobic soil actinomycete growing in thin hyphae and producing motile spores. In this PhD project the acarbose biosynthesis and function in Actinoplanes sp. SE50/110 are analyzed on the level of the cytosolic, extracellular and membrane bound proteome using IEF/SDS-PAGE MALDI-TOF MS and PMF.

Proteome studies on the synthesis and mode of action of the medically important secondary metabolite acarbose from Actinoplanes sp. SE50/110

Publications

Schwientek, P, Wendler, S, Neshat, A, Eirich, C, Rückert, C, Klein, A, Wehmeier, UF, Kalinowski, J, Stoye, J, Pühler, A. (2012). Comparative RNA-sequencing of the acarbose producer Actinoplanes sp. SE50/110 cultivated in different growth media. J Biotechnol. 2012 Nov 8. doi:pii: S0168-1656(12)00694-3. 10.1016/j.jbiotec.2012.10.019

Wendler, S, Hürtgen, D, Kalinowski, J, Klein, A, Niehaus, K, Schulte, F, Schwientek, P, Wehlmann, H, Wehmeier, UF, Pühler, A. (2012). The cytosolic and extracellular proteomes of Actinoplanes sp. SE50/110 led to the identification of gene products involved in acarbose metabolism. J Biotechnol. 2012 Aug 31. doi:pii: S0168-1656(12)00613-X. 10.1016/j.jbiotec.2012.08.011.

Document


Sergej’s Project
PDF (5.5 Mb)