Marius Terfrüchte

Project

Expression of malaria blood-stage proteins in Ustilago maydis

Info

Area: expression
Project Start: 1.08.2013
Supervisor(s): Prof. Dr. Karl-Erich Jaeger, Prof. Dr. Michael Feldbrügge, Dr. Kerstin Schipper
University: Duesseldorf

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Marius works at the Institute for Microbiology, Heinrich-Heine-Universität Düsseldorf.

Short project description

Many proteinaceous medicals are generated by microbial expression systems, linking industrial biotechnology closely to the pharmaceutical industry. Since a broad range of molecules can only be produced by microorganisms, there is a need for a variety of expression systems with specific qualities.

A very recent member of these organisms is the phytopathogenic fungus Ustilago maydis. Former studies revealed that U. maydis uses an unconventional, mRNA-transport coupled pathway which circumvents N-glycosylation to export the endochitinase Cts1.

To exploit this feature for the production and secretion of heterologous proteins in U. maydis, proteins of interest are fused to the Cts1 protein. Potential malaria-antigens, different antibody formats as well as several biopharmaceutical relevant proteins are examples for currently investigated targets.

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Marius’ project
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