Prakash Chandra Rathi


Enhancing protein thermostability without compromising enzyme activity by mutagenesis at sites predicted by rigidity analysis.


Area: biocatalysis, expression
Project Start: 1.04.2010
Supervisor(s): Prof. Dr. Karl-Erich Jaeger, Prof. Dr. Holger Gohlke
University: Duesseldorf


Dr. Rathi currently works for a pharmaceutical company in the UK. He received his doctoral degree from Heinrich Heine University Düsseldorf in October 2014.


Rathi PC, Pfleger C, Fulle S, Klein DL, Gohlke H (2011) Statics of biomacromolecules. In Modeling of Molecular Properties, Comba P (ed) pp 281-299. Weinheim: Wiley-VCH

Rathi PC, Radestock S, Gohlke H (2012). Thermostabilizing mutations preferentially occur at structural weak spots with a high mutation ratio. J. Biotech 159(3): 135-44

Krueger D, Rathi PC, Pfleger C, Gohlke H (2013). CNA webserver: Rigidity theory-based thermal unfolding simulations of proteins for linking structure, (thermo-)stability, and function. Nucl. Acid Res. 41: W340-348.

Pfleger C, Rathi PC, Klein DL, Radestock S, Gohlke H (2013). Constraint Network Analysis (CNA): A Python software package for efficiently linking biomolecular structure, flexibility, (thermo-)stability, and function. J. Chem. Inf. Model. 53:1007-1015

Rathi PC, Hoeffken HW, Gohlke H (2014) Quality matters: Extension of clusters of residues with good hydrophobic contacts stabilize (hyper)thermophilic proteins. J. Chem. Inf. Model. 54: 355–361.


Project description
PDF (114.9 kb)

Prakash´s CV
PDF (26.1 kb)