Anita Loeschcke

Project

A novel system for the functional expression of clustered genes

Info

Area: expression
Project Start: 1.05.2009
Supervisor(s): Prof. Dr. Karl-Erich Jaeger, Dr. Thomas Drepper, Prof. Dr. Martina Pohl
University: Duesseldorf

More

Dr. Anita Loeschcke was a fellow of the CLIB-Graduate Cluster from May 2009 until October 2012. She successfully defended her thesis on 24 October 2012 and is now a postdoctoral fellow at HHU Düsseldorf within the CEPLAS Cluster of Excellence on Plant Sciences, working on synthetic microbes for the production of plant secondary metabolites.

About the project

Microorganisms offer an immense variety of complex and/or functionally coupled biocatalysts that gain a steadily increasing importance in various biotechnological processes. For instance, high-value natural compounds and secondary metabolites are commonly synthesized in biocatalytic multi-step processes whose genes are naturally clustered in large chromosomal regions. However, the concerted functional expression of many genes that are located in large gene clusters using conventional expression hosts is hampered by various limitations. In order to overcome these limitations, the novel “in vivo auto cloning and expression system” (IVAC) was developed.

The challenge
Functional expression of clustered genes in common expression hosts as E. coli is hampered by various limitations:

  • Inefficient gene transcription due to the loss of functionality of promoter structures and/or regulatory elements in the heterologous expression host
  • Difficulties in the stable maintenance of imported gene clusters within the heterologous host due to vector instability
  • Problematic handling of large gene clusters

The answer to the problem
In order to overcome the described hindrances we developed the IVAC system, which allows the simultaneous functional expression of many genes that are grouped in a complex gene cluster in a broad range of different expression hosts.

PDF - 543.3 kb
Anita’s project







Publications

Arvani S, Markert A, Loeschcke A, Jaeger K-E, Drepper T (2012) A T7 RNA polymerase-based toolkit for the concerted expression of clustered genes. J Biotechnol; Accepted 2012

Loeschcke A, Markert A, Wilhelm S, Wirtz A, Rosenau F, Jaeger KE, Drepper T (2013) TREX: A Universal Tool for the Transfer and Expression of Biosynthetic Pathways in Bacteria. ACS Synthetic Biology 2: 22-33.

Short CV

Since 11/2012
Senior Staff at Institute of Molecular Enyzme Technology

05/2009 - 10/2012
CLIB-Graduate Cluster Fellow

12/2008 – 04/2009
Research associate at Institute for Molecular Enzyme Technology, Heinrich-Heine-University Düsseldorf, campus Forschungszentrum Jülich, (Germany).

12/2008 | Diploma in Biology (Dipl.-Biol.) | University Cologne
Institute for Molecular Enzyme Technology, Heinrich-Heine-University Düsseldorf, campus Forschungszentrum Jülich, Germany
Thesis: Functional expression of coupled genes using the IVAC system.
Supervisor: Dr. Thomas Drepper

09/2005 | Vordiplom, University Cologne

10/2003 - 02/2009
Study of biology at University Cologne, Germany

Documents


Anita’s project
PDF (543.3 kb)

Anita’s CV
PDF (21.8 kb)