Irene Kullartz

Project

Rational design of aldolases for organic synthesis

Info

Area: biocatalysis
Project Start: 1.05.2009
Supervisor(s): Prof. Dr. Holger Gohlke, Prof. Dr. Jörg Pietruszka
University: Duesseldorf

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Dr. Irene Kullartz was a fellow of the CLIB-Graduate Cluster from May 2009 until October 2012. She successfully defended her thesis on 25 October 2012 and now works for imat uve GmbH in Mönchengladbach.

Publications

I. Kullartz, J. Pietruszka, J. Biotechnol.; ’Cloning and characterisation of a new 2-deoxy-d-ribose-5-phosphate aldolase from Rhodococcus erythropolis’ J. Biotechnol. (2012) Oct 15;161(2):174-80

Kullartz I, Pietruszka J (2012) Cloning and characterisation of a new 2-deoxy-D-ribose-5-phosphate aldolase from Rhodococcus erythropolis. J Biotechnol; http://dx.doi.org/10.1016/j.jbiotec...

About the project

Naturally occurring active agents with often interesting physiological properties have been of huge interest in all times. Several cytotoxic agents could be isolated from marine organisms that are considered to be potential anti‐tumour agents. The families of bryostatins and pederins are two interesting classes of natural products which were actually isolated from marine organisms. An efficient access to the key structures of Bryostatin and Psymberin is desirable.

The focus of the project lies on the central tetrahydropyran (THP) which can be found as a substructure of both natural products. An efficient synthesis should be achieved by chemo‐enzymatic methods using aldolases. However, the desired key structure may not be accessible by using a wild type aldolase because of it’s stereoselectivity and in addition, the usage of a sterically challenging aldehyde as the substrate. Using directed evolution, the wild type enzyme should be adapted to its particular duty. The variants should be obtained by an enzyme screening based on fluorescence.

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Irene’s project








Short CV

07/2008 | Master | University of Duesseldorf
Institute for Bioorganic Chemistry, University of Duesseldorf, campus Forschungszentrum Jülich, Germany
Thesis: Aldolases in natural product synthesis: Development of a screening system.
Supervisor: Prof. Dr. Jörg Pietruszka

07/2006 | Bachelor | University of Duesseldorf
Institute for Organic Chemistry and Macromolecular Chemistry, University of Duesseldorf, Germany
Thesis: Contributions to the synthesis of functionalised o-Nitrobenzyl-compounds for the assembling of bichromophoresystems.
Supervisor: Priv.-Doz. Dr. Klaus Schaper

10/2003 - 07/2008 Study of biochemistry at University of Duesseldorf, Germany

02/2003 - 09/2003 Employment as chemical laboratory worker at the IMAT-UVE GmbH Mönchengladbach, Germany

07/1999 - 01/2003 Apprenticeship as chemical laboratory worker at the IMAT-UVE GmbH Mönchengladbach, Germany

Documents


Irene’s project
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Irene’s CV
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