Prof. Dr. Joachim Jose

University

department: Dept. of Pharmacy, Institute of Pharmaceutical and Medical Chemistry

More

University of Muenster, Department of Pharmacy, Institute of Pharmaceutical and Medical Chemistry (Head of the Institute), Bacterial Surface Display Group

Research interests

  • Evolutive drug design by surface display library screening
  • Biocatalytic synthesis of drugs or building blocks by whole cell biocatalysts with surface displayed enzymes
  • Test assay development using whole cells of E. coli with surface displayed target proteins

Among other systems for the secretion of proteins in Gram-negative bacteria, the autotransporter pathway represents a solution of impressive simplicity. The protein is transported, independent of whether it is a recombinant product or a natural passenger, as long as the coding region is introduced in frame between a typical signal peptide and the C-terminal domain referred to as the beta-barrel of the actual outer membrane translocation unit. The autodisplay system has been developed in an E. coli host background using the natural E. coli autotransporter protein, AIDA-I (adhesin involved in diffuse adherence). Autodisplay has been used for the surface display of random peptide libraries to successfully screen for novel enzyme inhibitors. The autodisplay system was also used for the surface display of functional enzymes, including esterases, oxidoreductases and electron transfer proteins. Whole E. coli cells displaying enzymes have been utilized to efficiently synthesize rare organic compounds of industrial impact with specific chirality. In addition, the cellular surface display of epitopes by autodisplay provided a novel way to raise immune protection by oral vaccination with attenuated Salmonella strains. Without disturbing cell integrity or viability, autodisplay facilitates the expression of more than 100,000 recombinant molecules per single cell, permits the multimerization of subunits expressed from genes encoding a monomer at the cell surface and allows the incorporation of an inorganic prosthetic group after transport of an apoprotein to the cell surface. The autodisplay system has been used for a broad spectrum of biotechnological applications including biocatalysis, assay development, tumor cell targeting, drug development and biosensor development.

To find out more, visit the website of the Institute of Pharmaceutical and Medical Chemistry or visit the Wikipedia page on Joachim Jose.


Students (co-)supervised

Stephanie Schumacher (alumna)
Agne Tubeleviciute