Jennifer Antfang

Project

Novel genetic tools for production strain development using the microbial cell factory Corynebacterium glutamicum as model

Info

Area: expression
Project Start: 1.06.2013
Supervisor(s): Prof. Dr. Michael Bott, Dr. Jan Marienhagen
University: Duesseldorf

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Jennifer works at the Forschungszentrum Jülich, Institute of Bio- and Geosciences, IBG-1: Biotechnology

Project overview

Development of producer strains requires the introduction of mutations into genomic DNA, which is currently done with plasmid-based methods. We succeeded to establish DNA recombineering in C. glutamicum with oligonucleotides in combination with biosensors to enable high-throughput screening via FACS. With this technique, termed RecFACS, it is possible to generate large targeted mutant libraries and to screen for productive mutants in a single experiment, establishing a new concept in synthetic biology.

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Jennifer’s project
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